BA-01M

Intracellular

Description

Features:

  • True current clamp operation with measured membrane potential and complete cancellation of series resistance
  • Can be used with sharp microelectrodes, and patch pipettes in the whole-cell and perforated-patch configuration
  • Digital DISPLAYS for current, voltage and electrode resistance
  • OSCILLATION SHUT-OFF unit prevents cells from damage
  • BUZZ and ELECTRODE CLEAR facility
  • Digital setting of gated stimulus amplitude, setting and digital read-out of holding current
  • Now with enhanced (x10) current range for electroporation and iontophoresis

Combine with

  • EXT-10C (extracellular amplifier module)
  • DPA-2FX (amplifier/filter module)
  • ISO-STIM 01M (stimulus isolator module)
  • TMR-02M (timer module)

and get a complete system for stimulating, extracellular and intracellular recording in one 19” rackmount cabinet

Details

The bridge amplifier module BA-01M is a wide bandwidth high impedance intracellular amplifier for recordings with sharp microelectrodes or suction (patch) pipettes. Currently, BA amplifiers are used in vertebrate (rat, mouse, cat, fish etc.) and invertebrate (leech, crab, snail, insect etc.) neurons, muscle cells, other excitable tissue and plant cells.

The BA-01M EPMS module has the same specification as the BA-01X 19" stand-alone device except that it has only one current input range (1 nA/V), only one potential output (x10 mV), only one bridge balance range (0-1000 MΩ) and no oscillation shut-off.

Bridge amplifiers are also available as 19" stand-alone devices.

Technical Data

Headstage:
Input voltage range:    ±1000 mV
Operating voltage:    ±15 V
Enclosure:    Size: 23 x 70 x 26 mm, grounded
Holding bar:    Size: length 150 mm, Æ 8 mm
Electrode connector:    BNC with driven shield
Ground connector:    2.4 mm connector
REF connector (optional):    SMB connector
Input resistance:    >1013 Ω (internally adjustable)
Current range x1:    ±12 nA into 1 GΩ
Current range x10:    ±120 nA into 100 MΩ

Electrode parameter controls:
BIAS:    range ±200 pA, current adjustable with trim potentiometer
OFFSET:    range ±200 mV, ten-turn control
capacitance compensation:    range 0 – 30 pF, ten-turn control

Cell penetration:
overcompensation of capacitance compensation (BUZZ), duration ~3 – ~110 ms (dependent on the setting of capacitance compensation), timer controlled with linear control, accessible with remote switch

Electrode clear circuit:
Application of max. DC currents (-Imax or +Imax)

Bridge balance:
Adjustable with ten-turn control, 0-1000 MΩ

Electrode resistance test:
10 mV / MΩ obtained by application of square current pulses ±2 nA, display XXXX MΩ

Bandwidth and speed response:
Full power bandwidth (REL = 10 kW):    >10 kHz, rise time (10% – 90%)
(Square pulse, 1V pp)    40 µs (voltage), 30 µs (current) (REL = 100 MΩ)
25 µs (voltage and current) (REL = 5 MΩ)
 

Outputs:
Resistance:    50 Ω
Current x1:    BNC connector, sensitivity 1 nA / V, display XX.XX nA, resolution 10 pA
Current x10:    BNC connector, sensitivity 0.1 nA / V, display XXX.X nA, resolution 100 pA
Potential:    BNC connector, sensitivity x10 mV, display XXXX mV

Inputs:
Current x1:
Current stimulus input via BNC connector, sensitivity 1 nA / V
Step gate input via BNC connector, gated stimulus with digital control of current step size (X.XX nA). Max. step size: 9.99 nA, resolution: 10 pA; polarity selectable with toggle switch, holding current range: ±10 nA, adjustable with ten-turn control
Current x10:
Current stimulus input via BNC connector, sensitivity 10 nA / V
Step gate input via BNC connector, gated stimulus with digital control of current step size, (XX.X nA). Max. step size: 99.9 nA, resolution: 100 pA; polarity selectable with toggle switch, holding current range: ±100 nA, adjustable with ten-turn control

Dimensions: 
Front panel:    24 HP (121.5 mm) x 3U (128.5 mm)
Housing:    7” (175 mm) deep

Downloads

Manual:
BA-01M Manual

References:
BA Reference List