EPC 10 USB Single

Patch Clamp Amplifier

Description

HEKA is pleased to release the newest family of fully computer controlled patch clamp amplifiers. The EPC 10 USB amplifiers are available with one (EPC 10 USB), two (EPC 10 USB Double), three (EPC 10 USB Triple) or four (EPC 10 USB Quadro) amplifiers. The EPC 10 USB patch clamp amplifiers are successors of the revolutionary EPC 9 patch clamp amplifiers, which were first introduced in 1990, and the well known EPC 10 patch clamp amplifiers, which replaced the EPC 9’s in 2002. This hardware product line is fully integrated with HEKA’s new LIH 8+8 AD/DA interface. The built-in interface utilizes the latest USB 2.0 and high speed processing technologies without the need for a peripheral PCI card. The advanced integration of the EPC 10 USB with the interface and software minimizes total recording noise, eliminates compatibility problems and reduces additional equipment expenses and set-up time.

 

The digital control of all amplifier functions has been designed with two advantages in mind. Firstly, if a computer controls the functions of the amplifier, then the data acquisition program can access and store variables that describe all of the settings during an experiment. Secondly, computer control allows a number of operations to be automated.

 

These include automatic switching (e.g, switching between the settings for establishing a seal or those for single channel recording) and also the automatic adjustment of capacitance transients cancellation and series resistance compensation. In fact, digital control of every adjustable parameter in the amplifier circuitry is implemented, including the calibration adjustments.

 

The EPC 10 is a complete data acquisition system, which can be used with HEKA’s PATCHMASTER or TIDA software. A DLL (dynamic link library) is available to allow access of the system from a custom written application. The EPC 10 USB patch clamp amplifier, combined with a computer and PATCHMASTER or TIDA software is equivalent to a fully equipped setup, which includes a patch clamp amplifier, a digital storage oscilloscope, a variable analog filter, a sophisticated pulse generator, and a fully featured data acquisition and analysis system.

Applications

  • Low noise single channel recordings
  • Low noise whole-cell patch clamp recordings: voltage clamp and current clamp / LFVC
  • Loose patch recordings
  • Intracellular voltage recordings with high resistance electrodes
  • Field potential recordings with metal microelectrodes
  • Recording from artificial membranes (Bilayer recordings)
  • Study of Long Term Potentiation (LTP) and Long Term Depression (LTD)
  • Study of Exocytosis/Endocytosis or Synaptic transmission by:
    • Measurement of whole-cell membrane capacitance (PATCHMASTER)
    • Measurement of on-cell membrane capacitance (PATCHMASTER)
    • Detection of released substances (amperometry with e.g. carbon fibre electrodes
    • Detection of releases substances under a patch (patch amperometry)

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