Optogenetics is a biological technique which involves the use of light to control cells in living tissue, typically neurons, that have been genetically modified to express light-sensitive ion channels. Optogenetics is now an important research method in the neuroscience community.
ALA Scientific’s Light Stimulation Device (LSD-1) can be incorporated into existing electrophysiology setups where light sensitive ion channels are being studied. Optical stimulation over standard electrical stimulation techniques affords researchers the advantage of non-invasively stimulating targeting areas.
Key features of the LSD-1 include:
- LED intensity control via manual knob or via analog signal for computer control
- Easily changeable LED’s for different wavelength use
- Low-noise electronics for integration into electrophysiology setups
- Field illumination when light guide connected to scope optics
- No warm-up period needed
- Combine the LSD-1 with the OptoPatcher electrode holder to make a power optogenetic stimulator
The LSD-1 uses a single LED to enable the application of a narrow wavelength of light for use as illumination or stimulation of a sample or biological preparation. Using a liquid light guide, or fiber optic cable (sold separately), the LSD’s output can be aimed at a sample on a microscope stage for field illumination to reveal photofluors or other luminescent substances. The light guide can also be introduced to microscope optics by connection to an appropriate port on a microscope to provide field illumination.
The LSD-1 via a fiber optic cable can also be connected to the OptoPatcher electrode holder for stimulation through a patch pipette.
The intensity of the LED can be controlled manually by a dial on the top of the controller, or an analog signal can be connected to the side BNC to provide computer control of the intensity and on/off of the LED via data acquisition system.
If necessary, the LED can be changed to another wavelength via a simple procedure.
|Universal AC adapter
||Input 100 – 240 VAC 50/60 Hz
Output 6 VDC @ 2.5 A max output
|Power Fuse (5 x 20 mm)
||2.0 A Fast Blow 250V
|Analog voltage input
||0 – 5 VDC
||0°C to 65°C
|Enclosure (W x H x D)
||13.2 x 9.4 x 8.1 cm (5.20 x 3.7 x 3.2 in)
||0.36 kg (0.8 Lbs.)
*These are the wavelengths available at this time due to the LED manufacturer in a state of flux with regard to power and light output. New wavelengths will be listed as they become available.