pE-300white

CoolLED pE-Series

Description

The pE-300white is a compact and simple to use LED illumination unit.  It provides a broad spectrum of illumination,  covering the excitation bands of common fluorescent stains.  Operation is by a remote manual control pod with instant on/off and intensity control from 0-100%.  Remote control is available via USB or TTL trigger.

CoolLED-pE-300white-spectrum-700

The system comprises a pE-300white Light Source, control pod, and power supply.  It can be specified with a microscope adaptor for direct fit configuration or with a 3mm diameter liquid light guide if there is a need to keep the source of illumination remote from the microscope.  An optional pE-Universal Collimator and microscope adaptor can be selected for use with a liquid light guide.

As a replacement to a metal halide source on a confocal microscope, the liquid light guide configuration is particularly suitable for pre-aligning samples before scanning commences.  In this way, photo bleaching of the sample can be minimised when LED illumination is used.

An additional benefit of the pE-300white is that  stains can be viewed either individually or in combination, without filter cube changes.  This makes it ideal for use with multi-band filter sets as the screening process can be simplified when fewer filter cubes are used.  Independent control of the three LED wavebands means that the user can control the level of excitation of each fluorescent stain independently on a multi-stained sample, potentially removing the need for single band filter sets altogether. For further information on improved multi-stain testing, go to the Downloads tab.

Print

With an expected lifetime in excess of 25,000 on hours and a comprehensive range of microscope adaptors, the pE-300white can be fitted to most current and older microscopes and operate for many years without aligning or replacing bulbs. The result is a safe, convenient illumination system without any additional operating costs.

There are two pE-300white configuration options:

Direct-fit  for connecting to a microscopes – by selecting from a range of microscope adaptors  which covers all current and most older models.  A simple once only adjustment will allow optimisation to the optical path of the microscope.

Liquid Light Guide with a fixed 3mm diameter, liquid light guide.  An optional pE-Universal Collimator can be specified in conjunction with a microscope adaptor if required.

Features

The pE-300white offers:-

  • Clean bright illumination across the spectrum – excites common fluorescent stains
  • Specify for  existing single and multi-band filter sets – no need to buy new filters
  • Instant on/off  – No shutters required, no warm up or cool down
  • Simple to fit, simple to use – no alignment, a once only adjustment
  • Stable & repeatable – reliable and consistent results
  • Wide range of microscope adaptors – fits most microscopes
  • Precise intensity control in 1% steps (0-100%) – no ND filters required
  • Excellent uniformity over field of view – fixed and stable, no alignment necessary
  • Long lifetime – expected to exceed 25,000 hours of operating time

Specifications

DAPI can be excited at either 365nm or 400nm and microscopes typically have a filter set which allows one or other of these wavelengths to be transmitted.  To accommodate this, the pE-300white can be specified in one of two waveband configurations to match with the user’s existing filter sets within the microscope.

A microscope which is populated with a number of single band (“SB”) filter sets will typically have DAPI excitation at 365nm and a microscope with multi-band (“MB”) filter(s) has excitation at 400nm.  The user can specify the configuration which is appropriate for their microscope.

Power requirements
100-240V a.c. 50/60Hz, 2A

Power consumption
2W (standby)
80W (full white intensity)

Dimensions

pE-300white Light Source: 77mm(w) x 186mm(d) x 162(h) – Weight 1.4kg
pE-300white Control Pod: 88mm(w) x 125mm(d) x 37mm(h) – Weight 0.32kg
pE-300white Power Supply: 167mm(w) x 67m(d) x 35mm(h) – Weight 0.62kg

pE-Universal Collimator: 44mm(w) x 86mm(d) x 44mm(h) – Weight 0.17kg

Environment & Safety
LED products are more sustainable and energy efficient than conventional illuminators. CoolLED’s products have the following benefits:

  • Mercury-free
  • Energy Efficient: 80% less power
  • Long lifetime (25,000 operating hours)
  • No bulb replacements
  • Reduced risk of eye damage
  • Quiet operation
  • No special disposal regulations or issues

Warranty 
System = 12 months, LEDs = 36 months

Control

Control pod for operation and access to settings and usage history.

Manual:
Manual control for instant on/off and intensity control in 1% steps from 0 – 100%
Individual bands can be selected/deselected and controlled independently as desired

Remote:
Via single TTL for on/off control of selected channels using a BNC connection on the light source.
Triggering speed <20us

Light-Delivery

Direct-fit to a microscope
Specify from CoolLED’s range of epi-fluorescent or transmitted port microscope adaptors

Liquid Light-Guide
1.5m long, 3mm diameter light-guide.

Ordering

pE-300-W-D-SB-YYY-ZZ: pE-300white direct fit Light Source, exchangeable microscope adaptor to customer-specified microscope, remote manual control pod, and power supply. Single band filter set configuration
pE-300-W-D-MB-YYY-ZZ: pE-300white direct fit Light Source, exchangeable microscope adaptor to customer-specified microscope, remote manual control pod, and power supply. Multi band filter set configuration
pE-300-W-L-SB-ZZ: pE-300white Light Source for use with 3mm Liquid Light Guide.  Single Band filter configuration.  Includes light source, control pod & power supply
pE-300-W-L-MB-ZZ: pE-300white Illumination System – Multi-Band.  For use with 3mm Liquid Light Guide.  Multi band filter configuration.  Includes light source, control pod & power supply
pE-1906 1.5m long, 3mm diameter liquid light guide
pE-10400 pE-Universal collimator for use with a single liquid light guide. Requires additional microscope adaptor
pE-ADAPTOR-YYY Microscope Adaptor to customer-specified microscope

To specify microscope code (YYY) see Adaptors
To specify power cable (ZZ):  10 = Australia, 20 = Europe, 30 = UK, 40 = USA

Downloads

CoolLED pE-300white leaflet
CoolLED pE-300white Improved Multi-stain Testing

Accessories

pE-10400: pE-Universal Collimator for use with a single liquid light guide. Requires additional microscope adaptor
pE-ADAPTOR-YYY: Microscope adaptor to customer-specified microscope