CoolLED pE-Series



As the first company to introduce LEDs into fluorescence microscopy, CoolLED has developed a comprehensive understanding of the complexities of configuring and selecting filter sets for samples which have multiple stains.  These ’multi-band’ filters must have a corresponding absorption and emission band for each stain and the bands must be kept spectrally separate to ensure there is no overlap.

CoolLED’s innovation comes from recognizing that all the stains used in multi-band work can have their absorption bands divided into four separate groups across the spectrum due to the restricted bandwidth available.  This has allowed the development of a patent-pending, wavelength-grouping concept which makes it possible to deliver more power in an efficient four channel system.

Wavelength grouping graphic without logo

With its unique 16 selectable LED wavelengths, the pE-4000 ensures optimum compatibility with all single and multi-band filter sets.  The flexibility and extensive functionality of the pE-4000 broadens the range of illumination options in core facilities.

pe-4000_01   pE-4000-channel-table
DAPI/FITC/TRITC/Cy5 Quad filter excited by pE-4000 at matched wavelengths shown above


The compact pE-4000 Light Source houses all the LEDs, their combining optics, thermal management and control electronics.  A dual mode control pod gives manual control in either White or Advanced modes.  Light is delivered to the microscope by either a single liquid light guide (LLG) or fiber. LLGs can be used in conjunction with CoolLED’s pE-Universal Collimator which can accept a microscope adaptor from CoolLED’s extensive range.


The pE-4000 offers:-

  • Broadest LED spectrum available: 365-770nm – match LED peaks to existing microscope filters
  • Four channel wavelength-grouping design matches with all commercially available dual, triple, and quad filters
  • Individual control of selected LED wavelengths – less background, no filter wheel or shutters
  • Integrated with many imaging software packages – can be incorporated into all experimental parameters
  • Instant on/off – no shutters required, no warm up or cool down
  • Stable & repeatable – reliable and consistent results
  • Wide range of Microscope Adaptors – fits most microscopes
  • Precise intensity control in 1% steps (0-100%) – no ND filters required
  • Excellent uniformity over field of view – fixed and stable, no alignment necessary
  • Long lifetime – expected to exceed 25,000 hours of operating time


  • Pre-sets allowing lab manager to match white spectrum to existing filter cubes
  • Higher contrast images from matched-white spectrum
  • Simple to fit, simple to use
  • No training required


  • Individual LED wavelength selection
  • Rapid switching between LED wavelengths enables capture of high speed events
  • TTL & USB interfaces with imaging packages
  • Excitation filters can be fitted in optical path for controlled switching with no moving parts
  • Analogue inputs for dynamic intensity control
  • Optical feedback for applications requiring higher stability
  • Internal function generator for electrophysiology and optogenetics applications


Power values
LED Wavelengths are divided across 4 channels with each channel having individual control.

λ Power FWHM λ Power FWHM λ Power FWHM λ Power FWHM
365 nm 15 mW 12 nm 460 nm 240 mW 19 nm 525 nm 39 mW 34 nm 635 nm 124 mW 16 nm
385 nm 59 mW 11 nm 470 nm 192 mW 20 nm 550 nm 167 mW 85 nm 660 nm 159 mW 21 nm
405 nm 188 mW 18 nm 490 nm 56 mW 32 nm 580 nm 167 mW 85 nm 740 nm 37 mW 29 nm
435 nm 139 mW 16 nm 500 nm 59 mW 29 nm 595 nm 33 mW 15 nm 770 nm 16 mW 28 nm

Powers measured at sample plane of research grade microscope using 100% mirror in cube and 10X objectives. (Figures are typical values).  Note: wavelength settings 550nm and 580nm use single broad, high intensity peak (see spectrum under Description tab)

Power requirements
110-240V a.c. 50/60Hz, 2.5A

Power consumption
Standby (i.e. no LEDs on) : Max 7W
Single wavelength operation : Max 41W
Dual wavelength operation : Max 75W
Triple wavelength operation : Max 93W
Quad wavelength operation : Max 112W

pE-4000 Light Source: 150mm(w) x 220mm(d) x 260mm(h) – Weight 3.5kg
pE-4000 Control pod: 154mm(w) x 135mm(d) x 40mm(h) – Weight 0.95kg
pE-4000 Power Supply: 164mm(w) x 64mm(d) x 35mm(h) – Weight 0.58kg
pE-Expansion Box: 151mm(w) x 18mm(d) x 95mm(h) – Weight 0.34kg

Environment & Safety
LED products are more sustainable and energy efficient than conventional illuminators. CoolLED’s products have the following benefits:

  1. Mercury-free
  2. Energy Efficient: 80% less power
  3. Long lifetime (25,000 operating hours)
  4. No bulb replacements
  5. Reduced risk of eye damage
  6. Quiet operation
  7. No special disposal regulations or issues

System = 12 months, LEDs = 36 months


Dual function remote manual control pod for White mode or Advanced mode

Via USB for independent on/off and intensity control of each channel. Triggering speed <1ms
Via 4 TTL inputs for independent on/off control of each channel. Triggering speed <20us
Via single TTL for on/off control of manual or software selected channels
Via 4 analogue inputs 0-10V, 0-300kHz for dynamic control of intensity from external analogue signals

Synch Out:
4 TTL outputs for each channel – active high
1 TTL output for any channel – active high

Programmable interface:
4 TTL outputs for on/off control of peripherals (transmitted illuminators, stages etc.)
4 analogue outputs for intensity control of peripherals (can be programmed to mirror LED intensities for channel control) 0-10V full scale.

Function Generator:
Internally generated sine, pulse and ramps for each channel programmed via control pod.

Connectivity:USB (B type) for PC connection. All other TTL and Analogue inputs/outputs via 25way ‘D-type’ female connector (optional rear mounting pE-Expansion Box available for BNC connectivity).


The pE-4000 can be configured to deliver light to the microscope via a single liquid light guide or fiber.


pE-4000-L-SYS-ZZ: pE-4000 Light Source with manual control pod, and power supply for LLG delivery
pE-4000-F-SYS-ZZ: pE-4000 Light Source with manual control pod and power supply for Fiber delivery
pE-4000-EB25D: Rear mounting pE-Expansion Box for 25-way D-type to BNC connectivity
pE-4000-EFH-4 Set of four Excitation Filter holders (25mm dia.)
pE-1906: 1.5m long, 3mm diameter liquid light guide
pE-10400: pE-Universal collimator for use with a single liquid light guide. Requires additional microscope adaptor
pE-ADAPTOR-YYY Microscope Adaptor to customer-specified microscope

A range of fibers is available from CoolLED, go to Accessories tab
To specify microscope code (YYY), see Adaptors
To specify Power Cable (ZZ):  10 = Australia, 20 = Europe, 30 = UK, 40 = USA


CoolLED pE-4000 leaflet


pE-4000-EB25D: Rear mounting pE-Expansion Box for 25-way D-type to BNC connectivity


pE-4000-EFH-4: Set of four Excitation Filter holders (25mm dia.)
pE-4000-L-MODULE: Interchangeable Light Delivery Output module for LLG (3mm dia.)
pE-4000-F-MODULE: Interchangeable Light Delivery Output module for Fiber (SMA/FC fittings)


CoolLED’s pE-4000 illumination system can be configured to accept a fiber via either an SMA or FC connector for connecting a wide range of fibers.

The user can source the fiber independently or request supply from CoolLED by specifying:-

Diameter in um (AAAA)
Numerical Aperture in mm (0BB)
Length in metres (C)
Termination 1 i.e. SMA, FC or C60- cleaved 60mm bare (DDD)
Termination 2 i.e. SMA, FC or C60-cleaved 60mm bare (EEE)

Product Code: pE-FAAAA-0BB-C-DDD-EEE


Examples below:-

pE-F50-022-2-SMA-SMA: 50 um diameter, .22 Numerical Aperture, 2m long fiber with two SMA terminations
pE-F50-022-2-FC-C60 50 um diameter, .22 Numerical Aperture, 2m long fiber with one FC termination and one 60mm cleaved end