Intracellular Bridge Mode and Extracellular Amplifier

BA-03X

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Category: Intracellular Tags: amplifiers, bridge recording, electrophysiology rig, extracellular recording, in vitro recording, in vivo recording, intracellular recording, oocyte recording, patch clamp, patch clamp rig, single electrode voltage clamp, two elecrode voltage clamp, xenopus recording

Overview

The BA amplifiers are wide-bandwidth high impedance amplifier designed for intracellular current clamp recordings with sharp microelectrodes, in perforated or tight seal patch configurations as well as for electroporation and iontophoresis. Currently, npi BA amplifiers are used in vertebrate (rat, mouse, cat, fish etc.) and invertebrate (leech, crab, snail, insect etc.) neurons, muscle cells, other excitable tissue and plant cells.

Each amplifier of the BA series is housed in a 19″ rackmount cabinet and equipped with functions like two ranges for bridge balance, an oscillation shut-off circuit for protecting the preparation, two potential and two current ranges. The amplifier comes with a small headstage with a holding bar (or a mounting plate / dovetail adapter on request) and a BNC connector for the electrode holder, and can be attached directly to a micromanipulator.

The BA-01X is the standard bridge amplifier fot intracellular current clamp recordings. Having an extended current range (x10, i.e. +/. 120 nA max.) this amplifer is also suitable for electroporation of single cells for non-invasive, juxtacellular filling of cells with dyes or plasmids.
The BA-03X has additionally high and low pass filters for the recorded potenial, and a potential gain up to 1000. Therefore, it allows extracellular recordings as well.

A bridge amplifier is also available as module for the EPMS-07 system.

Most precise current clamp recording method
Easy to use cell penetration mode with sharp microelectrodes
Perform extracellular recording or cell staining without changing headstage or pipette
Compatible with any data acquisition system
A simpler verion of this amplifier is the BA-01X
For additional voltage clamp capability choose the ELC-03XS
Combine with our miniature headstages for in vivo recording

Last Updated on October 8, 2024

Features

True current clamp operation with direct measurement of membrane potential and complete cancellation of series resistance and stray capacitance
Can be used with sharp mircroelectrodes, or patch pipettes in whole-cell, cell-attached and perforated-patch configuration
Can be used for extracellular recordings of sub millivolt signals as well
POTENTIAL OUTPUT GAIN: 10, 20, 50, 100, 200, 500, 1k
POTENTIAL HIGHPASS filter: DC: 0.1, 0.3, 0.5, 1, 3, 10, 30, 50, 100, 200, 500, 600, 1k, 3k Hz
POTENTIAL LOWPASS filter: 20, 50, 100, 200, 300, 500, 700, 1k, 1.3k, 2k, 3k, 5k, 8k, 10k, 13k, 20k Hz
CURRENT OUTPUT SENSITIVITY: 0.1, 0.2, 0.5, 1, 2, 5, 10V / nA
Digital DISPLAYS for current, voltage and electrode resistance
BUZZ and ELECTRODE CLEAR facility, AUDIO monitor
OSCILLATION SHUT-OFF unit prevents cells from damage
TTL gates stimulus input with amplitude setting by 3-digit potentiometer and digital read-out of holding current
Enhanced (x10) current range for Juxtracellular recordings, electroporation or Iontophoresis

Last Updated on October 8, 2024

Specifications

HEADSTAGE	Input voltage range: ±1000 mV Operating voltage: ±15 V Enclosure: Size: 23 x 70 x 26 mm, grounded Holding bar: Size: length 150 mm, diameter 8 mm Electrode connector: BNC connector with driven shield Ground connector: 2.4 mm connector Input resistance: >10¹³ Ω Current range x1: ±12 nA into 100 MΩ Current range x10: ±120 nA into 100 MΩ
ELECTRODE PARAMETER CONTROLS	BIAS: ±150 pA in range x1 and x10, adjustable with ten-turn control OFFSET: ten-turn control, range ±200 mV CAPACITY COMPENSATION: range 0-30 pF, ten-turn control.
CELL PENETRATION	Overcompensation of capacity compensation (BUZZ), duration ~2 – ~50 ms (slightly dependent on the setting of capacitance compensation), timer controlled with linear control, accessible with remote switch
ELECTRODE CLEAR CIRCUIT	Application of max. DC currents (-I_max or +I_max),
OSCILLATION SHUT-OFF	Turns off current injection and capacity compensation, function indicated by red / green LED, disabled / off / reset switch, threshold set with linear control (0-1200 mV).
BRIDGE BALANCE	adjustable with ten-turn control, 0-100 MΩ and 0-1000 MΩ with toggle switch.
ELECTRODE RESISTANCE test	10 mV / MΩ obtained by application of square current pulses ±1 nA, display XXXX MΩ.
BANDWIDTH and SPEED of RESPONSE	Full power bandwidth (Re = 0): >30 kHz, rise time (10 % – 90 %) <10 µs (Re = 100 MΩ), <5 µs (Re = 5 MΩ).
OUTPUTS (BA-01X)	Impedance: 50 Ω; Current x1: sensitivity 0.1 V / nA, display XX.XX nA, resolution 10 pA; Current x10: sensitivity 0.1V / nA, display XXX.X nA, resolution 100 pA; Potential: BNC connector, sensitivity x10 mV or x100 mV, selectable with toggle switch, display XXXX mV.
OUTPUTS (BA-03X)	Impedance: 50 Ω; Current range x1: sensitivity 1 V / nA to 10 V / nA, selectable by rotary switch, (7 steps); display XX.XX nA, resolution 10 pA; Current range x10: sensitivity 0.1V / nA to 10 V / nA, selectable by rotary switch, (7 steps); display XXX.X nA, resolution 100 pA; Potential: BNC connector, sensitivity x10 mV to x1000 mV, selectable with rotary switch (7 steps), display XXXX mV.
CURRENT INPUTS RANGE x1	0.1 nA / V, 1 nA / V, inputs equipped with on / off toggle switch, gating input with digital control of current step size and a resolution of 10 pA, polarity selectable with toggle switch, holding current range ±10 nA with ten-turn control.
CURRENT INPUTS RANGE x10	1 nA / V, 10 nA / V, inputs equipped with on / off toggle switch, gating input with digital control of current step size and a resolution of 100 pA, polarity selectable with toggle switch, holding current range ±100 nA with ten-turn control.
POWER REQUIREMENTS	115 / 230 V AC, 25 W
DIMENSIONS	19″ rackmount cabinet, 19″ (483 mm) wide, 10″ (250 mm) deep, 3.5″ (88 mm) high

Last Updated on October 8, 2024

Downloads

Manuals:
BA-03X Manual

Brochures:
BA Brochure

Intracellular amplifiers

Last Updated on October 8, 2024

References

Last Updated on October 8, 2024

Ordering

Part No.	Description
BA-03X	BA-03X 19″ Bridge Mode Amplifier
BA-MODA	Cell Model /w Active Membrane Resistance for BA System
BA-MOD	Cell Model Circuit (passive) for BA System
BA-EH	Standard Micro-/Patch Electrode Holder
BA-EH-SET	Electrode Holder Set for BRAMP-System
BA-PRS	Remote Control f. PENETRATION Unit
BA-PRS-F	Remote Control f. PENETRATION Unit (foot)
BA-HS	Headstage for BA System
BA-MINI	Miniature headstage for BA-series amplifier. Many different configurations available for in-vivo or in-vitro use, housing and cable options. Please contact us for further information and prices.

Last Updated on October 8, 2024