Overview
ELC amplifiers are multi technique systems where researchers can combine traditional patch and microelectrode recording with electrical stimulation, dye application or single cell transfection. All ELC amplifiers enable an investigator to identify a single cell by its characteristic spike train and then electroporate or stimulate this cell in situ. Using the loosepatch clamp technique for recording and stimulation in slice or in in vivo preparations, allow investigations under more natural, i.e. non-invasive conditions. Experiments can be executed with minimal damage to individual cells including subcellular structures such as dendrites and axons. Since no gigaseal is required for these types of experiments, the same pipette can even be used for several cells. The ELC incorporates a unique headstage with a resistive-feedback circuit capable of functioning as follows:
- Patch Clamp headstage with pA sensitivity
- High-impedance electrometer with bridge balance capable of functioning as a conventional bridge amplifier
- Extracellular amplifier with highpass and lowpass filters and gain up to 1000
ELC amplifiers come in 2 versions:
- ELC-01X and the ELC-01MX (modular version) include a simple voltage clamp function for approaching a cell and forming a seal.
- ELC-03XS is a complete patch clamp amplifier for whole-cell and perforated patch with improved series resistance compensation based on abridge balance circuit.
Therefore, ELC-03XS amplifiers offer an unprecedented combination of capabilities with only one headstage compared to other commercial amplifiers with a more limited functional design. Researchers will find this amplifier an excellent and very cost effective solution for carrying out multiple recording techniques while using several stimulation protocols.
- Save money with this simpler version of the ELC-03XS
- Perform juxtacellular fillings and cell staining
- Have the precise current clamp of a bridge amplifier
- Take advantage of voltage clamp for approaching cells
- Also available as module for the EPMS-07 system
- A similar amplifier without voltage clamp is the BA-01X
- Combine with our miniature headstages for in vivo recording
Last Updated on October 10, 2024
Features
- Recording with patch, sharp, metal or carbon fiber electrodes
- In vivo recordings with miniature headstages
- Extracellular recordings with high gain
- Headstages with additional extracellular channels
- Juxtasomal filling of dyes or DNA
- Single cell stimulation
- Iontophoresis
- Intracellular recording in CC (bridge) mode with patch or sharp electrodes
- Telegraph lines for filters and gains
- Compatible to all major data acquisition systems
Last Updated on October 10, 2024
Specifications
| HEADSTAGE |
Input voltage range: ±12V
Operating voltage: ±15V
Enclosure: 23 x 70 x 26 mm, grounded
Headstage connector: 8-pole DIN connector
Electrode connector: BNC with driven shield
Ground connector: 2.4 mm connector
Reference electrode connector: SMB connector (optional)
Input resistance (CC): >1013Ω (internally adjustable)
Current range: ±120nA into 100 MΩ, other ranges possible |
| ELECTRODE PARAMETER CONTROLS |
BIAS range: ±100pA, current adjustable with trim potentiometer
OFFSET range: ±100mV, ten-turn control
CAPACITY COMPENSATION range: 0 – 30pF, ten-turn control |
| BRIDGE BALANCE |
0 – 100MΩ, adjustable with ten turn control |
| ELECTRODE RESISTANCE TEST |
Sensitivity: 1 mV/MΩ, by application
of square current pulses ±1nA
Display: 3½ digits, XXX MΩ, activated by spring-loaded switch
Bandwidth and speed response:
Full power bandwidth (rise time (10% – 90%))
>30kHz (R = 0) EL
<10μs (R = 100 MΩ) EL
<5μs (R = 5 MΩ) |
| OUTPUTS |
Output impedance: 50Ω
Max. voltage: ±12V
Current output: BNC connector, sensitivity V/nA
Current output sensitivity: Rotary switch, range: 0.1,1,10
Current display: 3½ digits, resolution 10pA
Potential output x1: BNC, sensitivity 1 mV/mV
Potential output: BNC, range 10, 100, 1000
Potential low-pass filter: 3-pole BESSEL filter
attenuation: -18 dB/octave, corner frequency: 2kHz
Potential high-pass filter: 1-pole filter
attenuation: -6 dB/octave, corner frequencies (Hz): 1, 10, 50, 100, 500 |
| INPUTS |
Input impedance analog: 100kΩ
Input range: ±12V
Input impedance digital (TTL): 10kΩ
Input range (TTL): 0-5V, LO: <0.8V, HI: >1V
Current stimulus input via BNC connector, sensitivity: 10 nA/V
Step gate input via BNC connector (TTL)
Gated stimulus with ten-turn control of holding current, resolution: 10pA, range: ±100nA or with ten-turn control of holding potential
in VC mode: resolution 1mV, range ±1V
in VCx10 mode: resolution 10mV, range ±10V
Polarity selectable by toggle switch
Voltage command input via BNC connector, sensitivity: /10mV |
| DIGITAL DISPLAYS |
Display mV/MΩ: 3½ digits, XXXXmV or XXXMΩ
Display current: 3½ digits, XX.XXnA |
Last Updated on October 10, 2024
Downloads
Last Updated on October 10, 2024
Ordering
| Part No. |
Description |
| ELC-03XS |
Universal Amplifier |
| ELC-DUAL |
Two ELC-03XS Universal Amplifiers for Double Cell Recordings |
| ELC-01X |
Amplifier for extracellular recording and electroporation |
| ELC-01MX |
ELC-01 Module for EPMS System |
| ELC-MOD |
Cell Model Circuit for ELC System (passive) |
| ELC-MODD |
Dual-Cell Model Circuit for ELC System (passive) |
| ELC-SEAL |
Surplus for Seal Resistance Test |
| ELC-PRS |
Remote Switch for Penetration Unit |
| ELC-HS |
ELC Headstage |
| ELC-SWI |
Surplus for switchable Headstage, x1/x100 |
| ELC-SWI-M |
Surplus for switchable Headstage for ELC-01MX, x1/x100 |
| ELC-MINI-HS |
Miniature Headstages, several models available, on request |
Last Updated on October 10, 2024
