Overview
The MS-OPC is a unique chamber that uses a funnel effect to channel perfusion solutions around an Oocyte in a very efficient manor. The Oocyte is placed at the bottom of the funnel, on the glass surface of the cover slip that forms the bottom of the chamber. Typically the Oocyte will adhere to the glass to some extent. The position of the Oocyte at the bottom of the funnel insures that it will be well bathed by the perfusion solution that is put into the chamber through the metal canula that is provided (19gage). Typically the canula is the infusion point, but it can be used in reverse, if a device such as an ALA Levelock™ is used to manage the solution outflow. If your suction line is simply tied to a vacuum source, then the canula should be used for infusion, and your suction line placed on the side of the funnel. (If you require adjustable holders for tubing with a magnetic base that can secure the chamber and the tools, please check out MS Tools from ALA scientific as well)
The funnel shape of the chamber helps to keep fluid levels constant by allowing an ever increasing space as the fluid rises. Also, the shape gives solutions space to spread out and dilute after application on the Oocyte when the next solution is infused at the bottom. In addition, the small volume created by the chamber at the bottom insures fast perfusion time, and the shape of the walls will give maximum axis for two recording electrodes.
Last Updated on January 27, 2022
