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OptoPatcher

  • Model: Optopatcher
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SKU: N/A Categories: Pipette & Electrode Holder, Specialized Techniques Tags: Ag/AgCl electrodes, electrode holders, faraday cage, magnetic microscope stage tools, pipette holders, reference electrodes, silver silver chloride electrodes, slice grids, vacuum pump, vibration isolation tables
  • Additional information
  • Overview
  • Downloads
  • References
  • Ordering

Additional information

Model

OPTOPATCHER-AXU, OPTOPATCHER-BNC

Overview

The Optopatcher provides unmatched accuracy in applying optical stimulation to an in-vivo patch-clamp protocol. The holder houses both an optical fiber and an electrode enabling simultaneous patch-clamp recording and optogenetic activation.

This is a significant advantage in non-slice protocols, such as in-vivo recording from intact animals, whole-cell patch-clamping, LFP recording, and spike/single-unit recording.

The optopatcher was developed by A-M Systems under the guidance of its inventors, Dr. Ilan Lampl and Dr. Yonatan Katz of the Weizmann Institute of Science in Israel. (J Neurosci Meth 214 (2013) 113– 117).

The Optopatcher is available with the most common connectors used on patch clamps: Axon’s Threaded Collar  and standard BNC connectors used by Heka, A-M Systems and npi electronics. It can be ordered to accept capillary glasses with diameters between 1.2 mm and 2.0 mm.

All Optopatchers include a starter fiber, replacement seals, a mating sleeve for the ferrule.  Replacement parts are also available.

ALA’s Light Stimulating Device, LSD-1, is the perfect cost-effective light source for the Optopatcher.  The LSD-1 mates directly to any of the Optopatcher models by a 1m long light cable. The powerful LED is strong enough to activate rhodopsins and other photofluors. Color specific LEDs are easily interchangeable by the user.

Last Updated on October 14, 2024

Downloads

LSD-1 Product Literature

LSD-1 Product Manual

Last Updated on October 14, 2024

References

Bonzanni M, Rouleau N, Levin M, Kaplan DL (2020) Optogenetically induced cellular habituation in non-neuronal cells. PLOS ONE 15(1): e0227230. https://doi.org/10.1371/journal.pone.0227230

Last Updated on October 14, 2024

Ordering

Part# Description
OPTOPATCHER-AXU
Fiber optic light guide electrode holder for Axon Universal headstages, specify glass OD
OPTOPATCHER-BNC
Fiber optic light guide electrode holder for Heka, npi and all BNC type headstages, specify glass OD

 

Last Updated on October 14, 2024

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