Combine the DigiStim Stimulator and the ELectroPORATOR to form a flexible single cell electroporation system
Single-cell Electroporation is a flexible method to transfect individual cells in cell culture or tissue.
Last Updated on February 3, 2021
The ELectroPORATOR is an amplifier providing voltage pulses of up to 100V and current pulses up to 10mA which are timed and shaped by an external function generator or data acquisition system. It features offset and capacity compensation and a classical headstage. An audio monitor for electrode resistance, current or voltage is useful for approaching cells and completes the functionality of this instrument.
The combination with ALA’s DigiStim Waveform Generator forms a fully independent single cell electroporation system.
With DiGiStim
Last Updated on February 3, 2021
Output modes:
voltage source, current source
selectable with rotary switch
Scaling:
Voltage output: x 0.2 V/V
x 2 V/V
x 20 V/V
Current output:
x 2 mA/V
x 200 µA/V
x 20 µA/V
Displays:
Potential:
XXX.X V
Current:
XXX.X µA in x20 µA/V scaling
XXXX µA in x200 µA/V scaling
XX.XX mA in x2 mA/V scaling
Resistance:
XXX.X MΩ
Power:
XXXX mW in all scalings (voltage output)
XX.XX µW in x20 µA/V scaling (current output)
XXX.X mW in x200 µA/V scaling (current output)
XXXX mW in x2 mA/V scaling (current output)
Trigger Unit:
Trigger Input front panel: TTL, active high, 10 kΩ, max. 2 kHz
Trigger Input rear panel (REMOTE): TTL, active low
Push button
Trigger Output front panel: TTL, active high, 50 Ω, length: 400 µs
Offset Ranges:
Potential: ±1 V
Current: ±400 nA in x20 µA/V scaling (current output)
Current: ±4 µA in x200 µA/V scaling (current output)
Current: ±40 µA in x2 mA/V scaling (current output)
Monitors Scaling:
Potential: 100 mV/V
Resistance: 1 V/MΩ
Current (depending on mode and scaling):
1V/mA in all voltage outputs
100 mV/µA in x20 µA/V scaling (current output)
10 V/mA in x200 µA/V scaling (current output)
1 V/mA in x2 mA/V scaling (current output)
Power: 10 V/W
Last Updated on February 3, 2021
DigiStim: 2 channel USB based multifunction waveform generator
ELP-02D: Single-cell electroporator
Last Updated on February 3, 2021
Last Updated on February 3, 2021
BrainDancer® 
