TEC-03X for Standard Oocyte Recording

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Overview

TEC-03X – Two-Electrode Voltage Clamp Amplifier for routine recordings from oocytes

Features:

  • The TEC-03X is based on the standard two electrode approach and is an ideal, easy-to-use system for recording from oocytes
  • Accurate and fast two-electrode voltage and current clamp (V/C) amplifier with PI-controller for studying large membrane currents
  • Differential potential registration and high-voltage current source output, automated electrode resistance test mode which can be used even with the electrodes impaled in an oocyte
  • Digital DISPLAYS for current, voltage and electrode resistance
  • Two-pole (optionally four-pole) BESSEL filter for current
  • No virtual ground needed for recording membrane currents
  • OSCILLATION SHUT-OFF unit prevents cells from damage
  • Standard current range is ±150 µA into 1 MOhm. Current headstages with selectable current ranges (x0.1, x1, x2, x5 or x0.1, x0.2, x0.5, x1) are also available
  • Easy operation with all major data acquisition systems, remote selection of MODE of OPERATION (CC, VC), telegraphing (monitoring) outputs for current sensitivity and filter
  • Optional: Built-in interface for data acquisition with CellWorks

Last Updated on October 10, 2024

Features

Feature TEC-03X TEC-05X TEC-10CX
VC-Error display – X X
PI-Controller X X X
Series Resistance Compensation X X X
Electrode Resistance Test X X X
Mode of Operation Switch Rotary switch Rotary switch Push buttons
Bridge Mode – X –
DHC mode – opt. –
Lowpass filter: 20 … 20 kHz Two-Pole BESSEL
(opt. Four-Pole)		 Two-Pole BESSEL
(opt. Four-Pole)		 Four-Pole BESSEL
Direct Current output from Headstage X X X
Oscillation Shut-off X X X
Transient Compensation – – X
Capacity Compensation X X X
Cap. Comp. of Current Headstage – X –
Slow Mode: VCcCC
(Sutor et al., 2003)		 – opt. –
Please note:
These are the available standard configurations.
If you have special requirements,
please contact us directly.

Last Updated on October 10, 2024

Specifications

MODES OF OPERATION:
RPel: Potential Electrode Resistance Test;
CC: Current Clamp Mode;
VC: Voltage Clamp Mode;
RCel: Current Electrode Resistance Test;
BR: Bridge Mode (TEC-05X);
DHC Mode (TEC-05X, option);
HEADSTAGES
Potential headstage:
Operating voltage ±15 V;
Size (approx.): 70x26x26mm, holding bar diameter 8 mm, length 150 mm;
Input resistance: >1013 Ω;
Differential input (for supression of bath potentials): cmr >80 dB.

Current headstage (15 V, TEC-05X)):
Operating voltage: ±15 V;
Size (approx.): same as potential headstage,
Input resistance: >1012 Ω (internally trimmable).

Current headstage (150 V):
Operating voltage: ±150 V (standard);
Size (approx.): 100x50x30 mm, grounded enclosure,
holding bar diameter 8 mm, length 10 cm (isolated from ground, standard system only);
Input resistance: >1012 Ω (internally trimmable);
Power dissipation: 6 W (standard)

FEATURES:
Oscillation Shut-off
Electrode Resistance Test
Proportional Integrator
Series Resistance Compensation
Transient Compensation (TEC-10CX)

OUTPUTS:
CURRENT OUTPUTS:
Uncompensated output signal: sensitivity 0.1 V/µA or 0.1 nA/V (TEC-05X), voltage range ±15 V;

Compensated/filtered output: sensitivity: 0.1 V…10 V/µA (V/nA for TEC-05X)
POTENTIAL OUTPUTS:
Potential electrode: two outputs, sensitivity x10 mV only (TEC-03X) or x10 mV and x40 mV (TEC-05X, TEC-10CX), voltage range ±15V;
Current electrode: sensitivity x10 mV, voltage range ±15 V;

INPUTS:  
CURRENT CLAMP (standard headstage):
Inputs: 1 µA/V, 0.1 µA/V with ON/OFF switches (TEC-10CX), 1 µA/V (TEC-03X), 1 nA/V, 0.1 nA/V with ON/OFF switches (TEC-05X);
input resistance >100 kΩ;
VOLTAGE CLAMP:
Inputs: :10 mV and :40 mV (TEC-05X, TEC-10CX) with ON/OFF switches, input resistance >100 kΩ;

CONTROLS:
MODE selection: rotary switches (TEC-03X, TEC-05X), or pushbuttons (TEC-10CX) , LED indicators;

remote selection by TTL inputs, software assessable (optional).
Current Electrode Parameter Controls:
Offset compensation: ten-turn control, ±1200 mV;
Capacity compensation (optional, TEC-05X): range 0 – 30 pF, ten-turn potentiometer.
Potential Electrode Parameter Controls:
Offset compensation: ±200mV, ten-turn control;
Capacity compensation: range 0 – 30 pF, ten-turn control.
Oscillation Shut off: disabled / off / reset switch, threshold set with linear control (0- 1200 mV).
Current Clamp: HOLD: X.XX µA (TEC-03X, TEC-10CX), X.XX nA, ten-turn digital control with -/0/+ switch, maximum 10 µA (TEC-03X, TEC-10CX) or 10 nA (TEC-05X);
BRIDGE balance (optional, TEC-05X systems): max. 10 MΩ or max. 100 MΩ (switch selected) with ten-turn digital control.
INTEGRATOR TIME CONSTANT: 200 µs – 2 ms, ten-turn control (TEC-03X) or control with ON/OFF switch (TEC-05X, TEC-10CX) ;

Voltage Clamp: HOLD: XXX mV, ten-turn digital control with +/0/- switch, maximum 1000 mV;

RISE TIME LIMIT: 0 – 0.2 ms;
GAIN: 10 µA/V – 10000 µA/V, ten-turn linear control;
INTEGRATOR TIME CONSTANT: 200 µs – 2 ms, ten-turn control (TEC-03X) or control with ON/OFF switch (TEC-05X, TEC-10CX) ;

OUTPUT CURRENT LIMIT5 (TEC-10CX): 0 – 100%, linear control;

OUTPUT FILTERS:
Current output: lowpass  filters with 16 corner frequencies (20 Hz – 20 kHz)

AUDIO MONITOR:
Pitch correlated with potential signal.

DISPLAYS:
potential display: XXX mV.
Electrode resistance display XXX MΩ.
current display: X.XX µA
VC error (TEC-10X)

POWER REQUIREMENTS:
115 V/230 V AC, 60 W (1.25 A/0.63 A fuse, SLOW).

DIMENSIONS:
19″ rackmount cabinet, 19″ (483 mm) wide, 14″ (355 mm) deep, 5.25″(132.5 mm) high
weight: approx. 8 kg.

Last Updated on October 10, 2024

Downloads

Manuals:
TEC-03X Manual

Brochure:
TEC Brochure

 

Last Updated on October 10, 2024

References

Schmidt, A., Joussen, S., Hausmann, R. et al. Bile acids are potent inhibitors of rat P2X2 receptors. Purinergic Signalling 15, 213–221 (2019). https://doi.org/10.1007/s11302-019-09657-2

Isomerization of Asp7 in Beta-Amyloid Enhances Inhibition of the α7 Nicotinic Receptor and Promotes Neurotoxicity

Kryukova, E. V., Egorova, N. S., Kudryavtsev, D. S., Lebedev, D. S., Spirova, E. N., Zhmak, M. N., Garifulina, A. I., Kasheverov, I. E., Utkin, Y. N., & Tsetlin, V. I. (2019). From Synthetic Fragments of Endogenous Three-Finger Proteins to Potential Drugs. Frontiers in pharmacology, 10, 748. https://doi.org/10.3389/fphar.2019.00748

Last Updated on October 10, 2024

Ordering

Part No. Description
TEC-Oocyte Oocyte Complete Basic System: TEC-03X amplifier, INT-20X-USB interface, Xenoplace chamber system with 2x MM-33 Micromanipulator, VC3-4PG Perfusion System, Vacuum Waste Kit, Olympus SZ51 Stereoscopic Zoom Microscope, Pipette Holders
TEC-10CX TEC-10CX System
TEC-10X TEC-10 System for oocytes, without transient compensation
TEC-05-X TEC-05X system for high res. Electrodes, C.Comp. for current electrode, bridge mode, BUZZ, 4-pole Bessel Filter
TEC-03X TEC 03X System
TEC-DHC Dynamic Hybrid Clamp (DHC) Modification of TEC Syst.
TEC-SLOW VCcCC Modification of TEC System (surplus)
TEC-HS-P TEC Headstage for Potential Electrode
TEC-CHS-4 Current Headstage with four Ranges (surplus)
TEC-CHS-4S Current Headstage for high resistance microelectrodes (surplus)
TEC-HS150 Standard- Headstage for Current Electrode with ±150 V Output
TEC-RS-R Additional Syst. for RS-measurement (19″ rackmount cabinet)
TEC-PRS Remote Control f. PENETRATION Unit (hand)
TEC-MOD Passive cell model for TEC Systems

Last Updated on October 10, 2024

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