Increase efficiency and lower the cost of patch pipette production by using ALA’s CPM-2 Coating and Polishing Microforge. The CPM-2 is designed to be a complete system for processing pulled patch pipettes. It is available as a kit that mounts on a microscope or as a complete system with an inverted microscope.
Key Features of the CPM-2 include:
- Convenient control of heat timing with foot-pedal switch
- Heating and air pressure controls conveniently located in one unit
- Coating and polishing without electrode removal
- Available as complete system that includes inverted microscope with parfocal optics or as a kit
Electrophysiologists polish patch pipettes because pipette pulling often leaves sharp surfaces that can damage delicate cell membranes. Coating of pipettes is often necessary with the excised patches or cell-attached configurations to reduce noise from pipette capacitance and dielectric loss1. Pressure polishing, a innovation in pipette processing, minimizes series resistance with small-tip pipettes to facilitate whole-cell recording of small cells2.
1 Hamill, O.P. et al. Improved patch clamp techniques for high-resolution recording from cells and cell free membrane patches. Pflugers Arch. 391, 85-100 (1981).
2 Goodman, M.B. & Lockery, S.R. Pressure polishing: a method for re-shaping patch pipettes during fire polishing. J. Neurosci. Methods. 100, 13-15 (2000).