Overview
The Closed Perfusion Chamber (MS-CPC) from ALA is designed for perfusion of cells cultured on coverslips during imaging experiments. The closed design enables control of solution application under sterile conditions.
The Closed Perfusion Chamber is designed for use in cell biochemistry, physiology, biophysics and imaging research. The MS-CPC holds a sample of living cellular material on a glass coverslip forming a sealed chamber with a second glass coverslip (cells are either cultured on the glass, or deposited there prior to closure of the chamber). The two coverslips are separated by an o-ring made from an elastomeric material. When the chamber is closed, the o-ring separates the two pieces of glass and defines the actual “space” of the chamber.
MS-CPC-FLAT
Last Updated on January 27, 2022
Details
The o-ring is perforated in several places enabling a tube to be inserted through one perforation to provide an inlet for perfusion. The fluid moves out of the space defined by the o-ring and the two coverslips through other perforations in the o-ring. Perforations are made with a pointy object such as a needle. The effluent can either flow out through another tube passed through the o-ring, or seep out through the holes in the o-ring. The effluent that emerges from the o-ring is collected in the space surrounding the o-ring but within the confines of the nest and the top ring. The effluent can be removed from this space with a small suction tube.
Last Updated on January 27, 2022
Specifications
MS-CPC Specifications:
35mm x 7mm Approx.
O-ring: Standard is 12mm ID x 1.75mm high. (Square profile.)
Unit works best with 25mm round cover glass. Other sizes can be substituted so long as viewing hole supports the size. (Specifications subject to change.)
MS-CPC-FLAT Specifications:
O-ring: #19 Buna-quad profile
Cover Glass: #1 25mm
Dimensions: 76.5L x 30.5W x 5.5H (mm)
Material: 316 Stainless Steel
Last Updated on January 27, 2022
Ordering
| MS-CPC |
Round Perforated O’Ring Closed Perfusion Chamber for imaging and perfusion |
| MS-CPC-Flat |
Low Profile closed perfusion chamber – standard microscope slide dimensions |
Last Updated on January 27, 2022
