Overview
The VC3-8xP Series from ALA includes 8 channel valve controlled pressurized perfusion systems that fulfill the basic requirements of the student lab but can also be optimized to fit the most demanding applications of patch clamp recording and/or imaging. All VC3-8xP Series systems provide electronic control of solution exchange which improves reproducibility over manual methods. These systems are designed to minimize electronic noise so sensitive electrophysiological recordings are not disturbed.
Key features of the VC3-8xP Series include:
- Choice of low-maintenance pinch valves or fast-response isolation
valves
- Customizable reservoir sizes and materials
- DAQ control via analog or TTL input
- Valve control through DAQ, Valve Commander software, or controller
- Optional spill sensor with automatic shutoff to protect microscope
optics
- Optional MilliManifold™ or Quartz MicroManifold® perfusion outlet options for low volume and rapid solution exchange applications
- Valve open and close timestamps via analog or digital output
Last Updated on April 26, 2024
Details
Each VC3-8xP Series system includes the following:
- Magnetic stand with 1 x pole
- Valve manifold with isolation or pinch valves and 5mL pressurized
syringe reservoirs
- VC3 electronic valve controller with a USB cable and Valve
Commander software
- DB9-CABLE controller to valve manifold cable
Each VC3-8xP Series system features a choice of valves:
- Pinch valves: The 3-way normally open and normally closed pinch
valves require minimal maintenance in long-term perfusion
experiments and have response times of 15-20 ms.
- Isolation valves: These 2-way normally closed isolation valves
are optimized for high-speed solution exchange. These valves are
completely isolated when closed and have response times of 3 to 4
ms.
Additional options include:
- Glass reservoirs for volatile solutions
- Spill sensor: used to detect spills around the microscope to prevent damage to expensive optics
- Breakout Box: BOB-8 for TTL trigger inputs or outputs
Need to be ordered separately:
- 8 x CF-1’s and QMM-4WT for fast focal applications
- MLF-8 and tubing for fast bath applications
Last Updated on April 26, 2024
Specifications
Valve Controller:
- Dimensions/Weight: 8″/20.32cm x 6″/15.24cm x 2.5″/6.35cm / 1.5lbs/0.68kg
- Power: 15VDC/3.15A
- Computer Control Input: Analog input 0.5V/valve[ TTL high 1 bit/valve; USB 2.0
- Computer Output Monitoring: Analog output 0.5V/valve; Sync TTL high; TTL high 1 bit/valve
- Manual Control: Membrane switch 1/valve; mom on/off or toggle on/off
Valve Manifolds:
- Pinch Valves: 12VDC/0.25A each- 3-way normally open or normally closed pinch valves
- Pinch Valve Opening Speed: 15 to 20 ms
- Pinch Valve ID Tubing: 0.020″/0.5mm
- Clippard Isolation Valves: 12VDC/0.25A each
- Isolation Valve Opening Speed: 1-2 ms
- Reservoirs : 5mL pressurized syringes with 3-way stop cock luer valves
Sample pressurized flow rate: 1mL in 9 minutes @ 10 PSI / 70kPa through QMM with 100μm ID tubes
|
Pinch Valve |
|
3 way |
NO/NC port |
NC port |
Response time |
15 to 20ms |
2 to 5ms |
Wetted area |
silicone tubing |
PTFE |
Voltage |
12V |
12V |
Power |
4.2W |
1W |
Last Updated on April 26, 2024
Accessories
-
- MLF-8: MilliManifold TM 8 channel perfusion outlet manifold
- QMM-8: Quartz MicroManifold® 8 channel perfusion outlet manifold for low dead-volumes
- Compression Fittings: adaptors for tubing of different outer diameters
- PR-10: 0-10 psi analog two-stage pressure regulator
- Microscope Stage Plate and Tool Holders: microscope stage insert and perfusion tool holders
- VWK: Vacuum Waste Kit
- LL-2: LeveLockTM Fluid Level Controller
- SFK: miscellaneous tube and luer fittings
- BOB-8: for TTL trigger inputs or outputs
Last Updated on April 26, 2024
Downloads
VC3-8xP Manual
VC3-8xP Product Literature –>VC3-8xP literature is being updated to reflect the new isolation valve options
VC3 software (32-bit)
VC3 1.0.1.5 software (64-bit)
Last Updated on April 26, 2024
References
Spatial Coupling Tunes NMDA Receptor Responses via Ca2+ Diffusion
Falk-Petersen, C.B., Tsonkov, T.M., Nielsen, M.S. et al. Discovery of a new class of orthosteric antagonists with nanomolar potency at extrasynaptic GABAA receptors. Sci Rep 10, 10078 (2020). https://doi.org/10.1038/s41598-020-66821-0
Baldin J-P, Barth D and Fronius M (2020) Epithelial Na+ Channel (ENaC) Formed by One or Two Subunits Forms Functional Channels That Respond to Shear Force. Front. Physiol. 11:141. doi: 10.3389/fphys.2020.00141
https://www.frontiersin.org/articles/10.3389/fphys.2020.00141/full?report=reader
Last Updated on April 26, 2024
Ordering
Part# |
Description |
ALA VC3-8PP |
8 Channel Pressurized Perfusion System: pinch valves, magnetic stand with pole, valve manifold, 5mL pressurized reservoirs, VC3-C valve controller and power supply, cable, and software. 8 x CF-1’s and QMM-8WT or MLF-8 and tubing not included and MUST be ordered separately. |
ALA VC3-8CLP |
8 Channel Pressurized Perfusion System: isolation valves, magnetic stand with pole, valve manifold, 5mL pressurized reservoirs, VC3-C valve controller and power supply, cable, and software. 8 x CF-1’s and QMM-8WT or MLF-8 and tubing not included and MUST be ordered separately. |
ALA VC3-8C |
8 channel VC3 electronic valve controller including power supply, cables and software |
ALA VC3-PS |
VC3 electronic valve controller power supply |
ALA QMM-8 |
Quartz MicroManifold® 8 to 1 channel perfusion outlet manifold with 100um or 200um ID (please specify) tubing and tip (other sizes available – please call or email to inquire) |
ALA QMM-8WT |
Quartz MicroManifold® 8 to 1 perfusion outlet manifold with 100um or 200um ID (please specify) tubing and tip (other sizes available – please call or email to inquire) and clear teflon tubing installed |
ALA MLF-8 |
MilliManifold TM 8 to 1 channel perfusion outlet manifold made of 500um ID polyimide tubing with 1/16″ barb fittings |
ALA 60mlRES |
60mL pressurized reservoir assembly with 3-way luer valve |
ALA 10mlRES |
10mL pressurized reservoir assembly with 3-way luer valve |
ALA 5mlRES |
5mL pressurized reservoir assembly with 3-way luer valve |
ALA PV |
Replacement pinch valve |
ALA CL |
Replacement Clippard isolation valve |
ALA DB9-CABLE |
Replacement VC3 controller to valve manifold cable |
ALA BOB-8 |
BNC breakout box for TTL triggers |
ALA VM-STAND |
Replacement valve manifold stand: magnetic base and 2 rods |
ALA VC38AIRMAN |
Replacement pressurized air manifold tubing set |
ALA SPILSNSR |
Replacement Lasso Spill Sensor with connector |
ALA FEP-1 |
4m clear Teflon tubing with 230um ID and 600um OD (for 100um ID QMM’s) |
ALA FEP-2 |
4m clear Teflon tubing with 380um ID and 760um OD (for 200um ID QMM’s) |
ALA CF-1 |
Compression fitting for PE-10/FEP-1 tubing to 1/16″ ID tubing |
ALA CF-2 |
Compression fitting for PE-20/FEP-2 tubing to 1/16″ ID tubing |
ALA TUBING-2 |
10′ 1/16″ ID and 1/8″ OD PVC tubing |
ALA TUBING-4 |
2′ silicone pinch valve tubing for pressurized flow |
Last Updated on April 26, 2024