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P-V Pump

High Speed Pressure Clamp

HSPC-2-SB

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Category: Specialized Techniques
  • Overview
  • Details
  • Specifications
  • Downloads
  • Ordering
  • Research Papers

Overview

The HSPC-2-SB is an easy-to-use device for generating arbitrary pressure waveforms for the study of mechanosensitive ion channels during patch clamp recording. It is also used to stimulate the inner hair cells in the ear canal. The device consists of a control unit and a small headstage.

If you study mechanosensitive channels or stimulate inner hair cells, then you need ALA’s High Speed Pressure Clamp, HSPC-2-SB. The HSPC-2-SB is the only commercial instrument that can generate reproducible and rapid pressure/vacuum steps. Adding the HSPC-2-SB and the PV-Pump accessory to any patch clamp rig creates a complete system for biophysical studies in this important area.

Last Updated on March 31, 2025

Details

The 2nd generation High Speed Pressure Clamp (HSPC-2SB) device is a closed loop pressure-control servo optimized to deliver vacuum and pressure at zero average flow. Although the device is targeted at patch clamp applications, response characteristics are fully adjustable for use in other experimental environments. It features a steady state pressure range of ±200 mmHg, hardware to prevent water entering the control valve, an external command input and a buffered output pressure monitor. Command response has been optimized for steps as large as +/-200 mmHg. Pressure rise time is amplitude dependent and varies from 6 to 8 ms for a 20 mmHg step to 15 ms to 20 ms for a 200 mmHg step approximately. Fall time is also amplitude dependent and varies from 6 to 8 ms for a 20 mmHg step to 15 ms to 20 ms for a 200 mmHg step approximately. There is an amplitude independent delay of 1 ms on both the rising and falling edges of the response. System noise measured at the pressure sensor output is +/-0.5 mmHg peak to peak.

HSPC-2-SB System Highlights

• Pressure and vacuum partitioned by small headstage that easily mounts near amplifier probe
• Simple connection to electrode holder transmits pressure/vacuum pulses
• Command input of 20mV/mmHg sets pressure
• Pressure output of 20mV/mmHg signal monitor or from LCD display
• Moisture sensor prolongs life of headstage
• Compatible with all major patch clamp hardware/software
• Improves consistency of establishing gigaseals and whole-cell configuration

Last Updated on March 31, 2025

Specifications

Max. Input Pressure/Vacuum +/-7psi; 362mmHg
Standard Output Pressure/Vacuum Range +/-200mmHg
Noise +/-10mV; +/-1mmHg
Power 9V dc, 1A fuse
Controller 2.9lbs/1.32kg – 8.5”/21.6cm x 7.5”/19cm x 4”/10cm
Headstage 0.5lbs/0.23kg – 3.75”/9.5cm x 1.75”/4.4cm x 1.75”/4.4cm
Typical Speed of Response 0 to 100mmHg jump in 12ms: 0 to 100% settling time
Command Input 20mV/mmHg
Command Input 20mV/mmHg
Monitor Output 20mV/mmHg
Set Point Control (Holding Pressure/Vacuum offset control) +/-200mmHg
Moisture Alarm Capacitance liquid detection sensor to protect valve

P-V Pump:

Voltage 115V AC or 230V AC
Maximum Airflow 0.12 cfm / 3 l/min
Maximum Vacuum 17.5 in”Hg / 592 mbar
Frequency 60 Hz / 50 Hz
Noise Leve 40 dB(A) Max. @ 1 meter
Recognition UL / CE
Dimensions 32cmx10cmx15cm ht.
Weight 3.4Kg

Last Updated on March 31, 2025

Downloads

High Speed Pressure Clamp and P-V Pump Literature

High Speed Pressure Clamp Manual

P-V Pump Manual

Last Updated on March 31, 2025

Ordering

HSPC-2-SB Complete pressure clamp system includes controller, piezo valve headstage and cable, misc. tubing, and fittings
PV-Pump Pressure / vacuum pumps with output tubing

Last Updated on March 31, 2025

Research Papers

The mechanosensitive channel YbiO has a conductance equivalent to the largest gated-pore

Complexes of vertebrate TMC1/2 and CIB2/3 proteins form hair-cell mechanotransduction cation channels

NOMPC ion channel hinge forms a gating spring that initiates mechanosensation

The Mechanosensitive Ion Channel Piezo Inhibits Axon Regeneration

Trabecular Meshwork TREK-1 Channels Function as Polymodal Integrators of Pressure and pH

Sphingomyelinase disables Piezo1 channel inactivation to enable sustained response to mechanical force

The Piezo2 ion channel is mechanically activated by low-threshold positive pressure

Disruption of Membrane Cholesterol Organization Impairs the Activity of PIEZO1 Channel Clusters

The Transfer Characteristics of Hair Cells Encoding Mechanical Stimuli in the Lateral Line of Zebrafish

Piezo-like Gene Regulates Locomotion in Drosophila Larvae

A hydrophobic gate in the inner pore helix is the major determinant of inactivation in mechanosensitive Piezo channels

Cryo-EM structure of the SARS-CoV-2 3a ion channel in lipid nanodiscs 

Genomic non-redundancy of the mir-183/96/182 cluster and its requirement for hair cell maintenance 

The Lectin LecA Sensitizes the Human Stretch-Activated Channel TREK-1 but Not Piezo1 and Binds Selectively to Cardiac Non-myocytes 

High-speed pressure clamp.

Dynamic regulation of mechanosensitive channels: capacitance used to monitor patch tension in real time

Hypoosmotic- and pressure-induced membrane stretch activate TRPC5 channels

Piezo proteins are pore-forming subunits of mechanically activated channels

Inhibition of Mitochondrial Division Attenuates Cisplatin-Induced Toxicity in the Neuromast Hair Cells

Last Updated on March 31, 2025

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