4 Channel Pressurized Perfusion Systems

REPLACED BY  VC+

CONTACT ALA FOR MORE INFORMATION

*ALA is still supporting the VC3 systems for the foreseeable future*

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Overview

The VC3-4xP Series from ALA includes 4 channel valve controlled pressurized perfusion systems that fulfill the basic requirements of the student lab but can also be optimized to fit the most demanding applications of patch clamp recording and/or imaging. All VC3-4xP Series systems provide electronic control of solution exchange which improves reproducibility over manual methods. These systems are designed to minimize electronic noise so sensitive electrophysiological recordings are not disturbed.

Key features of the VC3-4xP Series include:

    • Choice of low-maintenance pinch valves or fast-response isolation valves
    • Customizable reservoir sizes and materials
    • DAQ control via analog or TTL input
    • Valve control through DAQ, Valve Commander software, or controller
    • Optional spill sensor with automatic shutoff to protect microscope optics
    • Optional MilliManifold  TM  or Quartz MicroManifold ®  perfusion outlet options for low volume and rapid solution exchange applications
    • Valve open and close timestamps via analog or digital output

Last Updated on April 26, 2024

Details

Each VC3-4xP Series system includes the following:

  • Magnetic stand with 1 x pole
  • Valve manifold with isolation or pinch valves and 5mL pressurized
    syringe reservoirs
  • VC3 electronic valve controller with a USB cable and Valve
    Commander software
  • DB9-CABLE controller to valve manifold cable

Each VC3-4xP Series system features a choice of valves:

    • Pinch valves: The 3-way normally open and normally closed pinch valves require minimal maintenance in long-term perfusion experiments and have response times of 15-20 ms.
    • Isolation valves: These 2-way normally closed isolation valves
      are optimized for high-speed solution exchange. These valves are completely isolated when closed and have response times of 3 to 4 ms.

Additional options include:

  • Glass reservoirs for volatile solutions
  • Spill sensor: used to detect spills around the microscope to prevent damage to expensive optics
  • Breakout Box: BOB-8 for TTL trigger inputs or outputs

Need to be ordered separately:

  • 4 x CF-1’s and QMM-4WT for fast focal applications
  • MLF-4 and tubing for fast bath applications

Last Updated on April 26, 2024

Specifications

Valve Controller:

  • Dimensions/Weight: 8″/20.32cm x 6″/15.24cm x 2.5″/6.35cm / 1.5lbs/0.68kg
  • Power: 15VDC/3.15A
  • Computer Control Input: Analog input 0.5V/valve[ TTL high 1 bit/valve; USB 2.0
  • Computer Output Monitoring: Analog output 0.5V/valve; Sync TTL high; TTL high 1 bit/valve
  • Manual Control: Membrane switch 1/valve; mom on/off or toggle on/off

Valve Manifolds:

  • Pinch Valves: 4 x 12VDC/0.25A each- 3-way normally open or normally closed pinch valves
  • Pinch Valve Opening Speed: 15 to 20 ms
  • Pinch Valve ID Tubing: 0.020″/0.5mm
  • Clippard Isolation Valves: 12VDC/0.25A each
  • Isolation Valve Opening Speed: 3-4 ms
  • Reservoirs : 5mL pressurized syringes with 3-way stop cock luer valves

Sample pressurized flow rate: 1mL in 9 minutes @ 10 PSI / 70kPa through QMM with 100μm ID tubes

Pinch Valve
Isolation valve
3 way NO/NC port NC port
Response time 15 to 20ms 2 to 5ms
Wetted area silicone tubing PTFE
Voltage 12V 12V
Power 4.2W 1W

Last Updated on April 26, 2024

Accessories

    • MLF-4: MilliManifold TM 8 channel perfusion outlet manifold
    • QMM-4: Quartz MicroManifold® 8 channel perfusion outlet manifold for low dead-volumes
    • Compression Fittings: adaptors for tubing of different outer diameters
    • PR-10: 0-10 psi analog two-stage pressure regulator
    • Microscope Stage Plate and Tool Holders: microscope stage insert and perfusion tool holders
    • VWK: Vacuum Waste Kit
    • LL-2: LeveLockTM Fluid Level Controller
    • SFK: miscellaneous tube and luer fittings
    • BOB-8: for TTL trigger inputs or outputs

Last Updated on April 26, 2024

Downloads

Manual

VC3-4xP Manual

Product Literature VC3-4xP Product Literature –> VC3-4xP literature is being updated to reflect the new isolation valve options

Product Literature VC3 software (32-bit)

Product Literature VC3 1.0.1.5 software (64-bit)

Last Updated on April 26, 2024

References

Spatial Coupling Tunes NMDA Receptor Responses via Ca2+ Diffusion

 

Holman HA, Poppi LA, Frerck M and Rabbitt RD (2019) Spontaneous and Acetylcholine Evoked Calcium Transients in the Developing Mouse Utricle. Front. Cell. Neurosci. 13:186. doi: 10.3389/fncel.2019.00186

https://www.frontiersin.org/articles/10.3389/fncel.2019.00186/full

 

Baldin J-P, Barth D and Fronius M (2020) Epithelial Na+ Channel (ENaC) Formed by One or Two Subunits Forms Functional Channels That Respond to Shear Force. Front. Physiol. 11:141. doi: 10.3389/fphys.2020.00141

https://www.frontiersin.org/articles/10.3389/fphys.2020.00141/full?report=reader

Last Updated on April 26, 2024

Ordering

Part# Description
ALA VC3-4PP 4 Channel Pressurized Perfusion System: pinch valves, magnetic stand with pole, valve manifold, 5mL pressurized reservoirs, VC3-C valve controller and power supply, cable, and software. 4 x CF-1's and QMM-4WT or MLF-4 and tubing not included and MUST be ordered separately.
ALA VC3-4CLP 4 Channel Pressurized Perfusion System: isolation valves, magnetic stand with pole, valve manifold, 5mL pressurized reservoirs, VC3-C valve controller and power supply, cable, and software. 4 x CF-1's and QMM-4WT or MLF-4 and tubing not included and MUST be ordered separately.
ALA VC3-4C 4 channel VC3 electronic valve controller including power supply, cables and software
ALA VC3-PS VC3 electronic valve controller power supply
ALA QMM-4 Quartz MicroManifold® 4 to 1 channel perfusion outlet manifold with 100um or 200um ID (please specify) tubing and tip (other sizes available – please call or email to inquire)
ALA QMM-4WT Quartz MicroManifold® 4 to 1 perfusion outlet manifold with 100um or 200um ID (please specify) tubing and tip (other sizes available – please call or email to inquire) and clear teflon tubing installed
ALA MLF-4 MilliManifold TM 4 to 1 channel perfusion outlet manifold made of 500um ID polyimide tubing with 1/16″ barb fittings
ALA 60mlRES 60mL pressurized reservoir assembly with 3-way luer valve
ALA 10mlRES 10mL pressurized reservoir assembly with 3-way luer valve
ALA 5mlRES 5mL pressurized reservoir assembly with 3-way luer valve
ALA PV Replacement pinch valve
ALA L2V Replacement Clippard isolation valve
ALA DB9-CABLE Replacement VC3 controller to valve manifold cable
ALA BOB-8 BNC breakout box for TTL triggers
ALA VM-STAND Replacement valve manifold stand: magnetic base and 2 rods
ALA VC34AIRMAN Replacement pressurized air manifold tubing set
ALA SPILSNSR Replacement Lasso Spill Sensor with connector
ALA FEP-1 4m clear Teflon tubing with 230um ID and 600um OD (for 100um ID QMM’s)
ALA FEP-2 4m clear Teflon tubing with 380um ID and 760um OD (for 200um ID QMM’s)
ALA CF-1 Compression fitting for PE-10/FEP-1 tubing to 1/16″ ID tubing
ALA CF-2 Compression fitting for PE-20/FEP-2 tubing to 1/16″ ID tubing
ALA TUBING-2 10′ 1/16″ ID and 1/8″ OD PVC tubing
ALA TUBING-4 2′ silicone pinch valve tubing for pressurized flow

Last Updated on April 26, 2024

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